Synthetic microbiology practicals

Synthetic microbiology practicals
Master BiotechnologiesParcours Biotechnologie synthétique (BS)

Description

Objectives in terms of knowledge (course content) :
Principles and methods to detect and differentiate various Candida species.
Detection and differential of skin bacteria.
Environmental microbiology: detection of fecal contamination, microbial degradation of oil spills.
Bacteriophage methods.
Genome engineering in human cell lines using CRISPR.
Elementary mathematical modeling of regulatory networks: the sigmoidal and bell-shaped responses.
Detection of single RNA molecules in cells.
 

Compétences visées

Objectives in terms of acquired skills :
The student will learn how to detect and differentiate bacteriophages, specific bacterial and fungal species, exemplified by Staphylococcus and Candida species. The student will acquire skills to modify genes in human cells using the bacterial CRISPR system. The student will be also able to write up, solve and analyze basic equations describing gene regulatory mechanisms