Description

Learning objectives (course content) :

• Plant hormones and mobilization of starch reserves in germinating grain
• Plant cell culture and plantlet regeneration
• Genetic engineering of seed content
• Induced resistance in wine grape and resveratrol production
• Transcriptomics and proteomics of stress responses in moss
• Transient expression of recombinant proteins in plant protoplasts
• In vivo calcium measurements
• Microinjection of plant cells
• Genetics of photoreceptors in plants
• Transformation of plants by particle bombardment and Agrobacterium infection
• Genetics of stem cell formation and maintenance in plants
• Epigenetic imprinting and reciprocal crosses
• Gene regulation by micro-RNAs
• Mapping a point mutation in Arabidopsis thaliana

Compétences visées

Objectives in terms of acquired skills :
(ie roughly what the student should be able to understand and apply in practical situations at the end of the course)

• To familiarize the student with genetics, physiology, genetic engineering and cell biology of plants.
• To foster scientific communication through seminars, critical review of literature and writing reports of experiments conducted during the course.

Bibliographie

References, books, course support :

• Lab manual of 220 pages.
• Scientific review and research articles.
• Lecture, tutorial and discussion periods