Advanced genetic engineering practicals
Diplôme d'ingénieur de l'École supérieure de biotechnologie de Strasbourg (ESBS)Parcours Diplôme d'ingénieur en biotechnologie
Description
1.Cloning of GFP by more complicated techniques:
- TA cloning
- Gibson cloning
- overlap extension PCR
- Golden Gate Cloning
2.Presentations by external specialists:
- GMO talk
- Golden Gate Cloning talk
- Crispr/Cas9 talk
Compétences visées
- Understanding the protocols used in complicated genetic engineering
- Acquiring the theory behind complicated clonings
- Acquiring a good practice in genetic engineering
- Being able to analyze and discuss experimental results
- Writting of a lab book
- Cleaning of the room and machines used
Bibliographie
Protocols and documents necessary to perform the experiments are given before the practical and all documents are available on Moodle.
Oral presentations are available in Moodle after the talk is done.