Genetic engineering practicals

Genetic engineering practicals
Diplôme d’ingénieur spécialité chimie-biotechnologies (Chembiotech)Parcours Diplôme d'ingénieur spécialité chimie-biotechnologies (Chembiotech)

ComposanteÉcole supérieure de biotechnologie de Strasbourg (ESBS)

Description

  1. Preparation of materials
  • Agar plates w/ antibiotics
  • Agarose gels
  • Competent cells
  1. Experimentation
  • Miniprep/Midiprep
  • Cloning of GFP into pUC19 (digestion, PCR, gel excision, ligation, transformation, colony picking)
  • Cloning of sgRNA into PX459 (digestion, annealing oligos, ligation, transformation, colony picking)
  • Verification of cloning by restriction digest
  • Colony PCR
  • RNA extraction
  • Reverse transcription
  • qPCR
  1. Analysis
  • Primer selection
  • Enzyme selection
  • Clonings
  • qPCR results

Compétences visées

  • Understanding the protocols used in genetic engineering
  • Acquiring the theoretical bases of cloning
  • Acquiring a good practice in genetic engineering
  • Being able to analyze and discuss experimental results
  • Writting of a lab book
  • Cleaning of the room and machines used


 

Bibliographie

Bibliography, course material : Protocols and documents necessary to perform the experiments are given before the practical and all documents are available on Moodle  

Contacts

Responsable(s) de l'enseignement