Description

1. Preparation of materials

• Agar plates w/ antibiotics
• Agarose gels
• Competent cells

1. Experimentation

• Miniprep/Midiprep
• Cloning of GFP into pUC19 (digestion, PCR, gel excision, ligation, transformation, colony picking)
• Cloning of sgRNA into PX459 (digestion, annealing oligos, ligation, transformation, colony picking)
• Verification of cloning by restriction digest
• Colony PCR
• RNA extraction
• Reverse transcription
• qPCR

1. Analysis

• Primer selection
• Enzyme selection
• Clonings
• qPCR results

Compétences visées

• Understanding the protocols used in genetic engineering
• Acquiring the theoretical bases of cloning
• Acquiring a good practice in genetic engineering
• Being able to analyze and discuss experimental results
• Writting of a lab book
• Cleaning of the room and machines used

Bibliographie

Bibliography, course material : Protocols and documents necessary to perform the experiments are given before the practical and all documents are available on Moodle  

Contacts

Responsable(s) de l'enseignement

• Anne-Laure Duchemin

MCC

Les épreuves indiquées respectent et appliquent le règlement de votre formation, disponible dans l'onglet Documents de la description de la formation.

Régime d'évaluation

CT (Contrôle terminal, mêlé de contrôle continu)

Coefficient

1.0

Évaluation initiale / Session principale - Épreuves

Seconde chance / Session de rattrapage - Épreuves