Genetic engineering practicals

  • Cours (CM) -
  • Cours intégrés (CI) -
  • Travaux dirigés (TD) -
  • Travaux pratiques (TP) 35h
  • Travail étudiant (TE) -

Langue de l'enseignement : Anglais

Description du contenu de l'enseignement

  1. Preparation of materials
  • Agar plates w/ antibiotics
  • Agarose gels
  • Competent cells
 
  1. Experimentation
  • Miniprep/Midiprep
  • Cloning of GFP into pUC19 (digestion, PCR, gel excision, ligation, transformation, colony picking)
  • Cloning of sgRNA into PX459 (digestion, annealing oligos, ligation, transformation, colony picking)
  • Verification of cloning by restriction digest
  • Colony PCR
  • RNA extraction
  • Reverse transcription
  • qPCR
  1. Analysis
  • Primer selection
  • Enzyme selection
  • Clonings
  • qPCR results

Compétences à acquérir

  • Understanding the protocols used in genetic engineering
  • Acquiring the theoretical bases of cloning
  • Acquiring a good practice in genetic engineering
  • Being able to analyze and discuss experimental results
  • Writting of a lab book
  • Cleaning of the room and machines used

 

Bibliographie, lectures recommandées

Bibliography, course material : Protocols and documents necessary to perform the experiments are given before the practical and all documents are available on Moodle  

Contact

Responsable

Anne-Laure Duchemin


Diplôme d’ingénieur chimie-biotechnologie "Chembiotech"