• Cours (CM) 10h
  • Cours intégrés (CI) -
  • Travaux dirigés (TD) 2h
  • Travaux pratiques (TP) 1h
  • Travail étudiant (TE) -

Langue de l'enseignement : Anglais

Description du contenu de l'enseignement

Objectives in terms of knowledge (course content):
  1. CM
    1. The mechanism of translation: what is needed for translation (components and actors), the process, differences in pro- and eukaryotes and what is the product of translation
    2. Post-translation: protein modification, trafficking, gene regulation, quality control
    3. Engineering: IRES, 2A peptides, fluorescent tags
    4. Methods to detect proteins: IHC/IF, purification, WB, IP/Co-IP, ChIP/ChIP-seq, Microscopy and FRET
  1. Group work
Students are divided into groups and work on a specific question related to translation process and its regulation. There is a session with the teacher in order to ask questions and guide the groups into the reflection on their subject. Afterwards, they write a little report (5 pages) and prepare a short presentation (10min) to explain their subject to the whole class.
  1. TD
Exercises about translation and application. There are 2 sessions: one for BT only and one for CBT only. This allows better participation of the students and give an example of the future examination exercises.
 

Compétences à acquérir

Objectives of the lessons :
  1. Understanding and acquiring of the mechanisms linked to the dynamics of gene expression in a cell (prokaryotes and eukaryotes) at molecular and cellular levels
  2. Knowing some techniques linked to proteins

    Bibliographie, lectures recommandées

    All documents necessary for the courses and TD are on Moodle.

     

    Contact

    École supérieure de biotechnologie de Strasbourg (ESBS)

    300, boulevard Sébastien Brant - CS 10413
    67412 ILLKIRCH-GRAFFENSTADEN
    0368854680

    Formulaire de contact

    Responsable

    Anne-Laure Duchemin


    Diplôme d’ingénieur chimie-biotechnologie "Chembiotech"