Pharmacogenetics

  • Cours (CM) 8h
  • Cours intégrés (CI) -
  • Travaux dirigés (TD) 4h
  • Travaux pratiques (TP) 20h
  • Travail étudiant (TE) -

Langue de l'enseignement : Français

Description du contenu de l'enseignement

Lectures content:
- types of genetic variability
- genetic variability and individual response to drugs (pharmacogenetics)
- new genotyping approaches to identify genetic variation (including new generation sequencing)

Practicals:
- Genomic DNA extraction from buccal cells and purification on a mini silica column
- Quantitative and qualitative characterization of genomic DNA by NanoDrop absorption spectrophotometry and Qubit spectrofluorometric measurements
- Genotyping reaction to search for Single Nucleotide Polymorphisms (SNP) -like polymorphisms affecting drug metabolism: use of pre-developed TaqMan-based assays for SNPs in genes encoding P450 cytochromes

Compétences à acquérir

Objectives in terms of knowledge:
Provide the student with knowledge allowing him:
- to understand the importance of genetic variations in drug development and use
- to apprehend current technological developments used to identify genetic variations
Objectives in terms of practical skills:
Perform genotyping of genetic variations and data analysis.

Objectives in terms of acquired skills:
Ability to:
- cite the different types of human polymorphism
- explain the principles of experimental methods used for genetic variation analysis presented during the course
- describe the importance of P450 cytochromes and polymorphism in their genes in differences in drug response between individuals
- establish, write and follow experimental protocols
- perform genotyping data analysis
- keep a laboratory notebook
- write a report in the form of a mini-publication (abstract, introduction, results and analysis, discussion and conclusion)
- present his/her experimental work orally using a PowerPoint presentation
 

Bibliographie, lectures recommandées

Scientific publications and other sources of information (protocols, websites) chosen by the teacher and illustrating the theme.
 

Contact

Faculté de Pharmacie

74 ROUTE DU RHIN
67401 ILLKIRCH-GRAFFENSTADEN
0368854110

Responsable

Maria Zeniou-Meyer


MASTER - Biotechnologies

Plaquette Master Biotechnologies