Projet in high throughput biotechnology - for French German Master

  • Cours (CM) -
  • Cours intégrés (CI) -
  • Travaux dirigés (TD) -
  • Travaux pratiques (TP) 75h
  • Travail étudiant (TE) -

Langue de l'enseignement : Anglais

Description du contenu de l'enseignement

Objectives in terms of knowledge (course content) :

The laboratory project will prepare students for a master thesis in plant biotechnology or related applied research. The students will work independently, supervised by experienced group members, on an individual research objective.
The following topics can be chosen from:
Molecular mechanisms of light perception and signalling (AG Hiltbrunner): Molecular mechanisms of light/phytochrome signalling; environmental signalling; molecular signal integration (e.g. light and temperature, light and phytohormones); evolutionary aspects of light signalling; expression and purification of phytochromes in bacteria, yeast, and/or mammalian cells; molecular characterization of purified phytochromes; reporter gene based genetic screen for light signalling mutants.
Biotechnology and synthetic biology of plant stem cells (AG Laux): Students will participate in a project that introduces them to stem cell biotechnology. They will work on a project to elucidate the regulation mechanisms of stem cell niches in response to environmental stresses and how changes in the stem cell niche secure the survival of plant organs in stress conditions. In a synthetic biology approach, the students will use strategies to enhance stem cell survival in adverse conditions. In a second synthetic biology project, students will learn how the control of stem cell regulators makes it possible to enhance plant regeneration and how somatic embryogenesis works, which is a widely used application to breed economically important plant cultivars.
Understanding and heterologous reconstitution of signalling hubs to engineer infection control modules in non-host species (AG Ott): Root nodule symbiosis is characterized by a tight molecular dialogue between the host and rhizobia. Entry into host cells is permitted in a strain-specific manner and is kept under stringent molecular control by the host. Successful colonization of host root cells results in an intracellula r release of the microbes into a newly developed organ, the root nodule that serves as a specified bioreactor. There, differentiated rhiziobia fix atmospheric nitrogen and deliver it to the host. This sustainable ‘natural fertilization system’ however is almost exclusively restricted to legumes. This course aims for sharpening the understanding of host cell infection with a focus on a symbiotic context. It is embedded in an international consortium effort that tries to engineer symbiotic nitrogen fixation in cereals. Our focus is a detailed understanding of an organizing centre that assembles infection related proteins and controls host cell entry.

Plant Biotechnology, Synthetic Biology, Signalling Research, Evo-Devo, Biopharmaceuticals (AG Reski): Functional genome or proteome analysis with Physcomitrella patens, Analysis of differential gene regulation, Principles of homologous recombination and gene targeting in Physcomitrella patens, Phytohormone action and developmental processes, cell cycle regulation in Physcomitrella patens, production of recombinant glycoproteins in Physcomitrella patens: glyco-engineering, gene expression, optimization of cultivation conditions, downstream processing.

Compétences à acquérir

Objectives in terms of skills

Molecular mechanisms of light perception and signalling (AG Hiltbrunner): The students can work on an experimental project, e.g. with Arabidopsis thaliana, with a focus on molecular biology, cell biology, plant physiology, or photobiology; find literature on a specific topic and extract the information relevant for the research question addressed in the lab; plan experiments to address a specific research question; apply the acquired methods independently; document and interpret the results scientifically correctly and discuss them in the scientific context; present the results in a research seminar
Biotechnology and synthetic biology of plant stem cells (AG Laux): The students will learn how to successfully plan and carry out experiments in plant stem cell biotechnology. The students will learn how to develop strategies to analyze stem cell regulation and how to apply the knowledge gained in a synthetic approach. The students will use modern microscopy techniques such as confocal microscopy, microfluidics and live cell imaging to analyze the spatial and temporal patterns of stem cell regulators. Students will obtain hands-on experience with plant regeneration, such as the production of somatic embryos.
Understanding and heterologous reconstitution of signalling hubs to engineer infection control modules in non-host species (AG Ott): The student will perform multi-component assemblies of de novo expression cassettes to co-express proteins that belong to the minimal core set that is required to trigger and control infection. He/she will use state-of-the-art expression systems (including Golden Gate Cloning, opto-genetic switches, etc.) and live cell imaging to study the assembly of the organizing centre in vivo. The candidate will express her/his genes of interests in homologous and heterologous plant backgrounds to test whether he/she can reconstitute a minimal infection site in a non-host species.
Plant Biotechnology, Synthetic Biology, Signalling Research, Evo-Devo, Biopharmaceuticals (AG Reski): The students can: work on an experimental project, e.g. with Physcomitrella patens, with a focus on molecular biology, cell biology or protein biochemistry or work on a theoretical project with a focus on bioinformatics; apply the acquired methods independently, document and interpret their results scientifically correctly and discuss them in the scientific context; reflect on the basic principles of advanced microscopy (confocal laser scanning microscopy) and to perform microscopic analyses under supervision.

MASTER - Biotechnologies

Plaquette Master Biotechnologies