TP Genetic engineering

  • Cours (CM) -
  • Cours intégrés (CI) -
  • Travaux dirigés (TD) -
  • Travaux pratiques (TP) 35h
  • Travail étudiant (TE) -

Description du contenu de l'enseignement

Objectives in terms of knowledge (course content) :

Different cloning methods :
  • cloning of a restriction fragment
  • cloning of a PCR fragment
  • cloning of a Tag
  • Golden Gate Cloning

RNA interference : ARN extraction, reverse transcription and Real Time PCR

CRISPRcas9 technology : detection of mutations
 

Compétences à acquérir

Objectives in terms of skills:
  • Acquire the principle of the protocols used in genetic engineering
  • Acquire a good practice in genetic engineering
  • Be able to analyse and discuss experimental results
  • Writting of a lab book

Contact

Ecole Supérieure de Biotechnologie de Strasbourg

300 BOULEVARD SEBASTIEN BRANT
67412 ILLKIRCH-GRAFFENSTADEN
0368854510


MASTER - Biotechnologies