TP Genetic engineering
- Cours (CM) -
- Cours intégrés (CI) -
- Travaux dirigés (TD) -
- Travaux pratiques (TP) 35h
- Travail étudiant (TE) -
Langue de l'enseignement : Français
Description du contenu de l'enseignement
Objectives in terms of knowledge (course content) :
Different cloning methods :
RNA interference : ARN extraction, reverse transcription and Real Time PCR
CRISPRcas9 technology : detection of mutations
Different cloning methods :
- cloning of a restriction fragment
- cloning of a PCR fragment
- cloning of a Tag
- Golden Gate Cloning
RNA interference : ARN extraction, reverse transcription and Real Time PCR
CRISPRcas9 technology : detection of mutations
Compétences à acquérir
Objectives in terms of skills:
- Acquire the principle of the protocols used in genetic engineering
- Acquire a good practice in genetic engineering
- Be able to analyse and discuss experimental results
- Writting of a lab book
Contact
Ecole Supérieure de Biotechnologie de Strasbourg
300 BOULEVARD SEBASTIEN BRANT67412 ILLKIRCH-GRAFFENSTADEN
0368854510
esbs-etudiants@unistra.fr
