TP Genetic engineering

Objectives in terms of knowledge (course content) :

Different cloning methods :

• cloning of a restriction fragment
• cloning of a PCR fragment
• cloning of a Tag
• Golden Gate Cloning

RNA interference : ARN extraction, reverse transcription and Real Time PCR

CRISPRcas9 technology : detection of mutations
 

Objectives in terms of skills:

• Acquire the principle of the protocols used in genetic engineering
• Acquire a good practice in genetic engineering
• Be able to analyse and discuss experimental results
• Writting of a lab book